High-Throughput siRNA Screens


At its most basic definition, siRNA and microRNA screening services (commercially available siRNA library screening services) enable researchers to search and screen tens of thousands of compounds of cellular-based and biochemical assays that help keep projects, experiments, and analysis on track. The ability to choose from a variety of validated assays within a number of target classes including ion channels and nuclear receptors provide high-quality data as well as quality controls on every plate. The screening process also enhances flexibility in accommodating cellular or biochemical assay technologies.

RNAi can be used as a tool to study functions of thousands of genes in a cell. siRNA libraries composed of large numbers of siRNAs have been developed. They are used for identifying sets of genes involved in a specific biological process or metabolism of a disease.

Phenotypic high throughput siRNA screens are a dominant approach to study relationships between structure and function of genes and to identify novel parts of signaling pathways.

In fact, large-scale siRNA screening is commonly used for functional genomic and drug target validation. High throughput applications requires that reliable and reproducible transfections be performed in 96-, 384-, or 1536-well plate format. Several transfection reagents are well-suited for high-throughput shRNA, siRNA, and miRNA transfection.

Genome-wide siRNA and microRNA screening service is commercially available from Altogen Labs high throughput library screening CRO service.

A technique called reverse transfection has been developed. It allows for plating of the cells and delivery of siRNA-reagent complexes on the same day (thereby eliminating a source of high degree of variability in cell cultures and transfection efficiency).

High-throughput protein expression analysis is now commercially available (Western Blot Inc) expanding screening technology capabilities.

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