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RNA Interference (RNAi)
Biochemical research have elucidated the mechanisms of RNAi. Double-stranded RNA is processed by enzyme (known as Dicer) resulting in production of the siRNA molecules. Those molecules can form a multi protein siRNA complex (known as the RNA-induced Silencing Complex (RISC)). The RISC/siRNA complexes catalyzes cleavages and degradations of complement mRNA molecules. The use of RNAi applications (including small interfering RNA) to reduce gene expression appear to have major advantages over other methods for targeting gene regulations. The potency of siRNAs, sequence specific design, and abilities of siRNAs to be reused to guide mRNA’s degradation in a cell bears significant advantages over antisense oligonucleotides and ribozyme approaches. Well designed functional siRNA are capable to effectively bypass the interferon response - in order to induce specific posttranscriptional gene silencing (or RNAi in vitro and in vivo). |
